Fig. 4: Clamshell dynamics of dimeric iGluR LBDs.

a ACFs, G(τ), normalized to the average number of molecules in the detection focus, recorded from GluA2-LBD-G446C-T685W-L483Y (apo monomer, red) and in presence of excess GluA2-LBD-L483Y (apo dimer, orange). ACFs of the dimer in presence of the agonists Glu and AMPA are shown in cyan and gray (agonist-bound dimers). b ACFs recorded from GluN1-LBD-A480C-N521Y (apo monomer, red) and in presence of excess GluN2A-LBD-E516Y (apo hetero-dimer, orange). The ACF of the GluN1-GluN2A hetero-dimer in presence of the agonist Gly is shown in cyan. Black lines are fits to the data using a model for molecular diffusion containing a sum of one to three single-exponential decays (indicated in (c, d)). c Amplitudes (closed bars) and corresponding time constants (open bars) of exponential decays of ACFs of the monomeric and dimeric GluA2 LBD in apo and agonist bound states. Data sets and color code correspond to the ACFs shown in panel (a). Crosses (X) denote missing (not detected) kinetics. d Amplitudes (closed bars) and corresponding time constants (open bars) of exponential decays of ACFs of the monomeric and hetero-dimeric GluN1 LBD in apo and agonist-bound states. Data sets and color code correspond to the ACFs shown in panel (b). Crosses (X) denote missing (not detected) kinetics. Error bars are s.d. of three measurements (n = 3).