Fig. 1: Low-percentage, low-viscosity Matrigel for suspension culture of patient-derived colorectal organoids and tumoroids. | Communications Biology

Fig. 1: Low-percentage, low-viscosity Matrigel for suspension culture of patient-derived colorectal organoids and tumoroids.

From: Low-viscosity matrix suspension culture enables scalable analysis of patient-derived organoids and tumoroids from the large intestine

Fig. 1: Low-percentage, low-viscosity Matrigel for suspension culture of patient-derived colorectal organoids and tumoroids.The alternative text for this image may have been generated using AI.

a–b Representative bright-field images and quantification of live-cell yield for (a) organoids and (b) tumoroids from three patients grown from single cells in media supplemented with 0, 3, 5, or 10% of Matrigel matrix (25,000 cells, 1 ml of medium/well) over a 14-day incubation period; scale bars, 200 μm. Data are plotted as mean ± s.d. Statistical significance was attributed to values of p < 0.05 as determined by the Student’s t test. NS, p > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001. N normal, T tumor.

Back to article page