Fig. 7: Scalable expansion of colorectal organoids and tumoroids in bioreactor tubes. | Communications Biology

Fig. 7: Scalable expansion of colorectal organoids and tumoroids in bioreactor tubes.

From: Low-viscosity matrix suspension culture enables scalable analysis of patient-derived organoids and tumoroids from the large intestine

Fig. 7: Scalable expansion of colorectal organoids and tumoroids in bioreactor tubes.

a Image of Corning™ Mini Bioreactor Centrifuge Tubes used for culture expansion. b Representative images of organoids and tumoroids grown as LVM suspension cultures in 6-well plates or bioreactor tubes over a 14-day period; low-magnification images, scale bars, 500 μm; high-magnification images, scale bars, 200 μm. c Quantification of normal and cancer organoid growth in bioreactor tubes. A total of 200,000 cells were seeded in 7 ml LVM suspension culture medium per tube; two independent normal and three independent cancer organoid cultures were assayed in duplicate. d Representative images of normal and cancer organoids grown in bioreactor tubes for 4–8 weeks, resulting in substantial enlargement of organoids and/or organoid aggregates; scale bars, 200 μm. N, normal; T, tumor; LM, liver metastasis; LU, lung metastasis.

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