Fig. 1: Construction of a vindoline-producing yeast strain. | Communications Biology

Fig. 1: Construction of a vindoline-producing yeast strain.

From: Efficient production of vindoline from tabersonine by metabolically engineered Saccharomyces cerevisiae

Fig. 1

a Metabolic pathway for the biosynthesis of vindoline/vindorosine from tabersonine. Eight plant-derived enzymes should be functionally expressed in yeast to reconstitute the vindoline biosynthetic pathway. T16H2: tabersonine 16-hydroxylase 2; 16OMT: 16-hydroxytabersonine O-methyltransferase; T3O: tabersonine 3-oxygenase; T3R: tabersonine 3-reductase; NMT: 3-hydroxy-16-methoxy-2,3-dihydrotabersonine-N-methyltransferase; D4H: desacetoxyvindoline-4-hydroxylase; DAT: deacetylvindoline-4-O-acetyltransferase; CPR: cytochrome P450 reductases; 16-OH-tabersonine: 16-hydroxytabersonine; 16-MOH-tabersonine: 16-methoxytabersonine; 3-OH-16-MOH-2,3-2H-tabersonine: 3-hydroxy-16-methoxy-2,3-dihydro-tabersonine; 3-OH-2,3-2H-tabersonine: 3-hydroxy-2,3-dihydro-tabersonine. b Brief engineering strategies for the construction of an efficient S. cerevisiae cell factory for high-yield production of vindoline from tabersonine. Different colors represented CPRs with different origins, e represented the electron transfer between CYPs and CPRs.

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