Table 1 Strains and plasmids used in this study.
Plasmid/strain name | Description | Source |
|---|---|---|
Plasmids | ||
pRS41K-SpCas9 | CEN/ARS; G418; AmpR; TEF1p-SpCas9-TEF1t | |
p423-SpSgH | 2μ; HIS3; AmpR; SNR52p-SUP4t | |
p426-SpSgH | 2μ; URA3; AmpR; SNR52p-SUP4t | |
pESC-URA | 2μ; URA3; AmpR; GAL1p-MCS1-CYC1t; GAL10p-MCS2-ADH1t (NCBI Accession AF063585) | Agilent Technologies, Inc |
pESC-LEU | 2μ; LEU2; AmpR; GAL1p-MCS1-CYC1t; GAL10p-MCS2-ADH1t (NCBI Accession AF063849) | Agilent Technologies, Inc |
pESC-LEU2d | 2μ; LEU2d; AmpR; GAL1p-MCS1-CYC1t; GAL10p-MCS2-ADH1t (Addgene plasmid #20120) | |
pESC-HIS | 2μ; HIS3; AmpR; GAL1p-MCS1-CYC1t; GAL10p-MCS2-ADH1t (NCBI Accession AF063850) | Agilent Technologies, Inc |
pESC-LEU2d-T16H2-16OMT | 2μ; LEU2d; AmpR; GAL1p-T16H2-CYC1t; GAL10p-16OMT-ADH1t | This study |
pESC-URA-CrCPR-CrCYB5 | 2μ; URA3; AmpR; GAL1p-CrCPR-CYC1t; GAL10p-CrCYB5-ADH1t | This study |
pESC-HIS-NMT-T3R | 2μ; HIS3; AmpR; GAL1p-NMT-CYC1t; GAL10p-T3R-ADH1t | This study |
pESC-URA-T3O | 2μ; URA3; AmpR; GAL1p-T3O-CYC1 | This study |
pESC-URA-D4H-DAT | 2μ; URA3; AmpR; GAL1p-D4H-CYC1t; GAL10p-DAT-ADH1t | This study |
pESC-URA-AtCPR1-CrCYB5 | 2μ; URA3; AmpR; GAL1p-AtCPR1-CYC1t; GAL10p-CrCYB5-ADH1t | This study |
pESC-URA-GuCPR1-CrCYB5 | 2μ; URA3; AmpR; GAL1p-GuCPR1-CYC1t; GAL10p-CrCYB5-ADH1t | This study |
pESC-URA-GlCPR-CrCYB5 | 2μ; URA3; AmpR; GAL1p-GLCPR-CYC1t; GAL10p-CrCYB5-ADH1t | This study |
pESC-URA-MTR2-CrCYB5 | 2μ; URA3; AmpR; GAL1p-MTR2-CYC1t; GAL10p-CrCYB5-ADH1t | This study |
pESC-URA-SAM2-INO2 | 2μ; URA3; AmpR; GAL1p-SAM2-CYC1t; GAL10p-INO2-ADH1t | This study |
pRS423-ZWF1-GAPN | 2μ; HIS3; AmpR; PGK1p-ZWF1-CYC1t; TEF1p-GAPN-ADH1t | This study |
Strains | ||
BY4741 | MATa: his3∆1 leu2∆0 met15∆0 ura3∆0 | ATCC |
VSY001 | BY4741 with pRS41K-SpCas9 | This study |
VSY002 | VSY001-GAL1p-CrCPR-CYC1t-GAL10p-CrCYB5-ADH1t | This study |
VSY003 | VSY002-GAL1p-T16H2-CYC1t-GAL10p-16OMT-ADH1t | This study |
VSY004 | VSY003-GAL1p-NMT-CYC1t-GAL10p-T3R-ADH1t | This study |
VSY005 | VSY004-GAL1p-T3O-CYC1t | This study |
VSY006 | VSY005-GAL1p-D4H-CYC1t-GAL10p-DAT-ADH1t | This study |
VSY007 | VSY006/pESC-LEU2d-T16H2-16OMT | This study |
VSY008 | VSY006-GAL1p-T16H2-CYC1t-GAL10p-16OMT-ADH1t | This study |
VSY009 | VSY008-GAL1p-T3O-CYC1t-GAL10p-T3R-ADH1t- GAL1p-NMT-CYC1t- GAL1p-D4H-CYC1t-GAL10p-DAT-ADH1t | This study |
VSY014 | VSY009-GAL1p-T16H2-CYC1t-GAL10p-16OMT-ADH1t | This study |
VSY015 | VSY014-GAL1p-T16H2-CYC1t-GAL10p-16OMT-ADH1t | This study |
VSY016 | VSY015-GAL1p-T16H2-CYC1t-GAL10p-16OMT-ADH1t | This study |
VSY017 | VSY015-ΔCrCPR::AtCPR1 | This study |
VSY018 | VSY015-ΔCrCPR::GuCPR1 | This study |
VSY019 | VSY015-ΔCrCPR::GlCPR | This study |
VSY020 | VSY015-ΔCrCPR::MTR2 | This study |
VSY021 | VSY015-GAL1p-CrCPR-CYC1t-GAL10p-CrCYB5-ADH1t | This study |
VSY022 | VSY015-GAL1p-AtCPR1-CYC1t-GAL10p-CrCYB5-ADH1t | This study |
VSY023 | VSY017-ΔOPI:: PGK1p-ZWF1-CYC1t-TEF1p-GAPN-ADH1t | This study |
VSY024 | VSY023-GAL1p-SAM2-CYC1t-GAL10p-INO2-ADH1t | This study |
VSY017-2 | VSY017- GAL1p-AtCPR1-CYC1t-GAL10p-CrCYB5-ADH1t | This study |
VSY017-3 | VSY017-ΔNCP1::GAL1p-AtCPR1-CYC1t-GAL10p-CrCYB5-ADH1t | This study |
VSY017-4 | VSY017-ΔNCP1::GAL1p-GuCPR1-CYC1t-GAL10p-CrCYB5-ADH1t | This study |
VSY017-5 | VSY017-ΔNCP1::GAL1p-GlCPR-CYC1t-GAL10p-CrCYB5-ADH1t | This study |
VSY017-6 | VSY017-ΔNCP1::GAL1p-MTR2-CYC1t-GAL10p-CrCYB5-ADH1t | This study |
VSY017-7 | VSY017-ΔNCP1::GAL1p-CrCPR-CYC1t-GAL10p-CrCYB5-ADH1t | This study |
VSY025 | VSY024-ΔNCP1::GAL1p-AtCPR1-CYC1t-GAL10p-CrCYB5-ADH1t | This study |
