Fig. 3: PCYOX1 is abundant in human and Apoe−/− mice atherosclerotic lesions.
From: Prenylcysteine oxidase 1, an emerging player in atherosclerosis
Sections of human atherosclerotic lesions were subjected to IHC (a–d) or ISH (f–h) analysis for the detection of PCYOX1 protein and mRNA, respectively, and counterstained with hematoxylin. (a; objective ×2.5; b–d; objective ×40). e Representative image of the positive control probe (housekeeping gene, Ubiquitin C), demonstrating mRNA integrity (objective ×2.5). f–h PCYOX1 mRNA staining (red punctate dots, objective ×40). b, f luminal side; c, g lipid-rich regions; d, h tunica media. i–l PCYOX1 and apoB immunofluorescence in human atherosclerotic lesions counterstained with DAPI (confocal images, objective ×40). Shown are representative images from one donor. m–p Serial sections immunostained for PCYOX1 (m), apoB (n), and farnesyl groups deriving from isoprenylated proteins (o) or in the absence of primary antibody (negative control, p). Arrows indicate the internal elastic lamina. Shown are representative images from another donor. Negative controls are shown in Supplementary Fig. 2. q–t Aortic root sections from Pcyox1+/+/Apoe−/− and Pcyox1−/−/Apoe−/− mice fed a HFD for 8 weeks subjected to ISH for the detection of Pcyox1 mRNA alone (q, r objective ×40) or in combination with immunostaining for cell-type-specific protein markers (objective ×63), F4/80 for macrophages (s), and α-SMA for SMC (t). Tissue sections were counterstained with hematoxylin. Arrows in q–s indicate internal elastic lamina. Arrowheads in t indicate the expression of PCYOX1 in luminal ECs.
