Fig. 5: Deletion of htsH1, htsH2, and htsH3 resulted in decreased HSAF production.

a Quantification of the HSAF produced by the htsH1, htsH2, and htsH3 mutant strains and strains complemented with the htsH1, htsH2, and htsH3 genes grown in 10% TSB medium. b Growth curves of the htsH1, htsH2, and htsH3 mutant strains and strains complemented with the htsH1, htsH2, and htsH3 genes in LB-rich medium. c Growth curves of the htsH1, htsH2, and htsH3 mutant strains and strains complemented with the htsH1, htsH2, and htsH3 genes in 10% TSB medium. Error bars, means ± standard deviations (n = 3 biologically independent samples). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, assessed by one-way ANOVA. All experiments were repeated three times with similar results.