Fig. 7: HtsH1, HtsH2, and HtsH3 directly target plafB depending on their PDE activity. | Communications Biology

Fig. 7: HtsH1, HtsH2, and HtsH3 directly target plafB depending on their PDE activity.

From: The predatory soil bacterium Lysobacter reprograms quorum sensing system to regulate antifungal antibiotic production in a cyclic-di-GMP-independent manner

Fig. 7

a–c Phosphorylation analysis of HtsH1C-Flag-His, HtsH2C-HA-His, or HtsH3C-Myc-His with calf intestine alkaline phosphatase (CIAP) at concentrations ranging from 0.1 to 1.6 µg in the reaction mixtures (20 μL, 28 °C, 60 min) using Mn2+-Phos-tag SDS-PAGE. d–f Gel shift assay showing that HtsH1C-Flag-His, HtsH2C-HA-His, or HtsH3C-Myc-His does not directly target plafB with calf intestine alkaline phosphatase (CIAP) at concentrations ranging from 0.1 to 1.6 µg in the reaction mixtures (20 μL, 28 °C, 60 min). HtsHs (4 μg) were added to reaction mixtures containing 50 ng of probe DNA, and the reaction mixtures were separated on polyacrylamide gels.

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