Fig. 3: Visualization of NK-cells lytic granules after 5.5 h coculture with A. fumigatus hyphae. | Communications Biology

Fig. 3: Visualization of NK-cells lytic granules after 5.5 h coculture with A. fumigatus hyphae.

From: Subdiffraction-resolution fluorescence imaging of immunological synapse formation between NK cells and A. fumigatus by expansion microscopy

Fig. 3

Perforin (magenta) and granulysin (cyan) were visualized by antibody staining, fungal hyphae via RFP-expressing mitochondria (yellow). a Conventional CLSM images prior to expansion. Perforin and granulysin are partially overlapping in round-shaped granules. b ExM images. Post ~4x expansion, labeled perforin appears as a ring-like structure, whereas granulysin was also distributed throughout the NK cell. Insets in panel b represent a detailed view of the indicated region. c Effect of degranulation. In the direct environment of degranulating NK cells, mitochondria appear distorted or even absent (white arrow) in comparison to hyphae more distant (yellow arrow). All images represent maximum intensity z-projections. Dotted lines indicate borders of NK cells (gray) and fungal hyphae (yellow). Representative images of at least three biological replicates. Scale bars, 5 µm (a), 10 µm (b), and 25 µm (c).

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