Fig. 9: Expression patterns of ERL1, ER, and ERL2 in ovules and MMC specification phenotype in er-105 erl1-2 erl2-1 ovules. | Communications Biology

Fig. 9: Expression patterns of ERL1, ER, and ERL2 in ovules and MMC specification phenotype in er-105 erl1-2 erl2-1 ovules.

From: High-throughput single-cell transcriptomics reveals the female germline differentiation trajectory in Arabidopsis thaliana

Fig. 9: Expression patterns of ERL1, ER, and ERL2 in ovules and MMC specification phenotype in er-105 erl1-2 erl2-1 ovules.

a, b GFP signals were detected in ovules at different stages from AC to the post-meiotic stages. Expression patterns of pERL1: GFP (a); expression patterns of pER: GFP (b); expression patterns of pERL2: GFP (c). The magenta signal corresponds to FM4-64 dye outlining the ovule. Scale bars,10 μm. d–g DIC images of wild-type (WT) (d) and er-105 erl1-2 erl2-1 (e–g) ovules. Numbers denote the frequencies of the phenotypes shown. MMC is fake colored by yellow. Scale bar, 20 μm. h–k AGO9 immunolocalization of WT (h) and er-105 erl1-2 erl2-1 (i–k) ovules. Green and magenta signals correspond to AGO9 localization and propidium iodide signal, respectively. Scale bars,10 μm.

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