Fig. 4: GS3.1 functions as a MATE transporter and might transport p-coumaric acid in rice.
a Growth complementation assays of the E. coli ΔacrB mutant expressing GS3.1HJX on medium supplemented with the antibiotic norfloxacin. The E. coli ΔacrB mutant expressing pMAL empty vector and the wild-type E. coli BW25113 were used as control strains, and mediums without norfloxacin were used as the growth condition control. Scale bar = 1 cm. b A schematic of the predicted GS3.1 structure showing 12 transmembrane helixes. Numbers indicate the sequence number of amino acid residues. c Predicted GS3.1 model docked with p-coumaric acid (red). The α-helixes interacting with p-coumaric acid were colored purple. The amino residues interacting with p-coumaric were colored yellow. The red arrow indicates the key α-helix for its transporter function.
