Table 1 Cryo-EM data collection, refinement and validation statistics.
| Â | PCV-1 VLP |
|---|---|
Data collection and processing | Â |
Sample applications to grid | 1 |
Magnification | 75, 000 x |
Voltage (kV) | 300 |
Electron exposure (e–/Å2) | 66.11 |
Defocus range of micrographs (μm) | −0.5 to −2.0 |
Pixel size (Ã…) | 1.065 |
Symmetry imposed | I |
Initial particle images (no.) | 260, 000 |
Final particle images (no.) | 105, 000 |
Map resolution (Ã…) | 2.9 |
FSC threshold | 0.143 |
Number of frames | 59 |
Refinement | Â |
Map sharpening B factor (Å2) | −127.7 |
Model composition | Â |
 Protein residues | 668 |
 Nucleic acids | 0 |
R.m.s. deviations | Â |
 Bond lengths (Å) | 0.006 |
 Bond angles (°) | 0.664 |
Validation | Â |
 Clashscore | 8.29 |
 Poor rotamers (%) | 8.69 |
Ramachandran plot | Â |
 Favoured (%) | 91.36 |
 Allowed (%) | 8.18 |
 Disallowed (%) | 0.45 |
