Fig. 3: MRF7 C-terminal region affects Golgi localisation.
From: Arabidopsis thaliana myosin XIK is recruited to the Golgi through interaction with a MyoB receptor
a–c C-terminal fusion of GFP to ΔMRF7 localises to the cytosol, suggesting that the Golgi-targeting information could reside at the C-terminus of MRF7 (red asterisk). The C-terminal GFP fusion could be responsible for masking such signal, preventing a Golgi localisation. d–f Localisation of GFP-ΔMRF7ΔC reflects that of ΔMRF7-GFP, indicating that the last 54 amino acids are fundamental to target MRF7 to the Golgi. g Quantification of GFP-ΔMRF7ΔC Golgi localisation compared to that of GFP-ΔMRF7. Each datapoint represents one cell; values on the left-hand side indicate the percentage of Golgi in a particular cell with GFP-ΔMRF7 or GFP-ΔMRF7ΔC surface localisation. Data from 53 cells (GFP-ΔMRF7) and 51 cells (GFP-ΔMRF7ΔC) from 5 independent experiments, Mann–Whitney test, ****P < 0.0001. All imaging carried out two days post-infiltration. Scale bar = 5 μm.
