Fig. 4: Localisation of GFP-MRF7 and GFP-ΔMRF7 in A. thaliana is consistent with that observed in tobacco.
From: Arabidopsis thaliana myosin XIK is recruited to the Golgi through interaction with a MyoB receptor
A. thaliana lines stably over-expressing GFP-MRF7 (green) or GFP-ΔMRF7 (green) were generated by floral dipping of wild-type Col-0 plants. Stable lines from T4 generation were then crossed with a ST-mRFP (magenta) marker line. Progeny were imaged 14 days post-germination. a–c As in tobacco leaf epidermal cells (Fig. 2a–c), GFP-MRF7 localises both to puncta (filled arrowheads) and to the Golgi surface (empty arrowheads) in Arabidopsis leaf epidermal cells. Magnification in the top panel shows a detail of the Golgi localisation: GFP-MRF7 fluorescence is brighter on subdomains of the Golgi. d–f As in tobacco leaf epidermal cell (Fig. 2d–f) GFP-ΔMRF7 localises to the Golgi and in the cytosol in Arabidopsis leaf epidermal cells. Scale bar = 5 µm.
