Table 1 Average lifetime values, FRET efficiencies and estimated intermolecular distance for the FRET pairs tested.

From: Arabidopsis thaliana myosin XIK is recruited to the Golgi through interaction with a MyoB receptor

FRET pair

Ï„D (ns)

Ï„DA (ns)

EFRET (%)

r (nm)

GFP-MRF7 + RFP-XIK tail (*)

2.4 ± 0.1

2.3 ± 0.1

4.2

9.1

GFP-ΔMRF7 + RFP-XIK tail (*)

2.4 ± 0.1

2.4 ± 0.1

—

» 10

GRIP-GFP + RFP-ARL1

2.3 ± 0.1

2.1 ± 0.1

8.7

7.9

ST-GFP + ST-mRFP

2.4 ± 0.1

2.4 ± 0.1

—

» 10

  1. τD indicates the donor’s lifetime in the absence of the quencher ± SD, τDA indicates the donor’s lifetime in the presence of the quencher ± SD. Asterisks indicate combinations whose lifetimes were measured both on Golgi and in the cytosol. FRET efficiencies (EFRET) and estimated intermolecular distances (r) were calculated according to Eqs. (1) and (2) described in the Methods section.
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