Fig. 3: Naphthofluorescein attenuates Mint3-mediated glycolysis in MDA-MB-231 cells and macrophages.
From: Pharmacological inhibition of Mint3 attenuates tumour growth, metastasis, and endotoxic shock
a Mint3 expression in MDA-MB-231 cells transfected with siRNAs against green fluorescent protein (GFP; siGFP) and Mint3 (siMint3#1, #2). b Lactate production of control and Mint3 knockdown MDA-MB-231 cells treated with DMSO or naphthofluorescein (Naph; 10 μM). c V5-tagged Mint3 expression in MDA-MB-231 cells transfected with mock or V5-tagged Mint3 expression vector. d Lactate production of mock or V5-tagged Mint3 expressing MDA-MB-231 cells treated with DMSO or Naph (10 μM). e Mint3 expression in wild-type (WT) and Mint3 knockout (KO) bone-marrow-derived macrophages. f Lactate production of WT and Mint3 KO macrophages treated with DMSO or Naph (10 μM). g ATP levels of WT and Mint3 KO macrophages treated with DMSO or Naph (10 μM). In (b), (d), (f), and (g), error bars indicate SD (n = 3). Data were analysed using Student’s t test. *p < 0.05, **p < 0.01. NS not significant.
