Table 2 Capture and detection of LAM from the urine of subjects with pulmonary TB by Sandwich ELISA.

From: Monoclonal antibodies from humans with Mycobacterium tuberculosis exposure or latent infection recognize distinct arabinomannan epitopes

Diagnosisa

Microbiology

U-LAMb ELISA OD450

U-LAM Detectionc

GC-MS (ng/mL)d

 

Smeare

Culturef

CS35/A194

L1AM04/T1AM09

  

Pulmonary TB

pos

Mtb

1.649

1.531

Positive

25

Pulmonary TB

pos

Mtb

0.894

0.627

Positive

14.3

COPD, Pneumonia

neg

neg

0.233

0.210

Negative

Not detected

Pneumonia, TB unlikely

neg

neg

0.196

0.221

Negative

Not detected

  1. aAll four patients were HIV uninfected.
  2. bCapture ELISA for the detection of LAM in urine (U-LAM).
  3. cU-LAM detection above cut-off based on background values for healthy control urine for each mAb pair by capture ELISA (OD450 0.259 for CS35/A194 mAb pair and 0.335 for L1AM04/T1AM09 mAb pair, respectively).
  4. dQuantification of U-LAM by gas chromatography-mass spectrometry (GS-MS) D-Arabinose detection method.
  5. eSputum smear microscopy for acid fast bacilli (AFB).
  6. fMycobacterial culture of sputum.
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