Fig. 3: Adipogenic plasticity is evident in adipose tissue-derived stromal/stem cells (ASCs), not human adipose tissue-derived microvascular endothelial cells (HAMVECs).

HAMVECs and ASCs were cultured in adipogenic medium for 10 days before their accumulation of lipids was assessed by Oil Red O with hematoxylin counterstaining. Endothelial medium is used as control. Shown are representative photomicrographs depicting the accumulation of lipids by HAMVECs and ASCs (a), as well as a bar graph delineating its quantification (b). Hematoxylin stains nuclei blue/purple, and Oil Red O stains lipids red/orange. Scale bars represent 100 µm. Values represent mean ± standard deviation; and *p < 0.05. Experiments were performed in biological triplicate, using cells derived from three different donors (n = 3 biologically independent samples).