Fig. 2: Timecourse and proposed mechanism of RNC crosslinking. | Communications Biology

Fig. 2: Timecourse and proposed mechanism of RNC crosslinking.

From: An intrinsically disordered nascent protein interacts with specific regions of the ribosomal surface near the exit tunnel

Fig. 2: Timecourse and proposed mechanism of RNC crosslinking.The alternative text for this image may have been generated using AI.

a SDS-PAGE analysis and corresponding plot illustrating the timecourse of EDC-mediated PIR RNC crosslinking. See Methods section for details. b Proposed mechanism of PIR RNC crosslinking and rate constant (k1; avg ± S.E., for n = 2) with corresponding lifetime (τ1) value derived from data fitting. Gray, orange, and black arrows denote steps that were not explicitly modeled, burst-phase steps, and explicitly-modeled slow steps, respectively. c Fractions of conformationally dynamic and static RNC populations assessed via fluorescence anisotropy decay22 (light and dark blue, respectively), and fractions of non-crosslinked and crosslinked PIR RNCs upon 30 min (light and dark gray, respectively) exposure to EDC. Data are displayed as avg ± SE (n = 2). d Kinetic simulations employing, as input, the rate constants derived from fitting of the data in panel a. See Fig. S2a–c for additional simulations.

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