Fig. 4: Intravital tracking of quiescent ISCs and “differentiation-poised” EBs in the intestines of young and healthy flies. | Communications Biology

Fig. 4: Intravital tracking of quiescent ISCs and “differentiation-poised” EBs in the intestines of young and healthy flies.

From: Intravital imaging strategy FlyVAB reveals the dependence of Drosophila enteroblast differentiation on the local physiology

Fig. 4

a Detection of ISCs and EBs over 3 days with the interval of 1 day. A distinctive cell pattern (solid line) formed by four ISCs (S1-S4, white arrowheads) was re-identified over time. An ISC-EB-EB cluster (ISC, EB1, and EB2, dashed square in the first panel) and an ISC-EB pair (ISC and EB4, dashed square in the second panel) were analyzed at two individual z-sections. Newborn EB3 were identified at day 3. Magnification and schematic illustration inside the dashed squares are shown in the upper right corner. b Detection of ISCs and EBs over 4 days with the interval of 2 days. An ISC-EB-EB cluster (ISC, EB5, and EB6, dashed box1) and two single ISCs (dashed box2 and box3) were detected using the pattern of ISCs (S1–S5). Newborn EB7 and EB8 were identified at day 2. Magnification and schematic illustration inside the dashed squares are displayed in the upper right corner and lower panels. c Detection of ISCs and EBs over 2 days with the interval of 1 day. A putative ISC-EB pair (ISC and EB9, dashed box) was detected using the pattern of ISCs (S1–S5). NRE-GFP in EB9 (white asterisk) was still visible at day 2. Magnification and schematic illustration inside the dashed squares are shown in the upper right corner. Genotype in all images: esg-Gal4 10×UAS-myr:tdTomato, NRE-GFP. All scale bars in a–c are 10 μm. d The quantification of GFP ratio for EBs from a–c (except for EB3). Measurement of the GFP fluorescence intensity of an EB and its surrounding BG, and Notch activity at different time points were normalized using the “signal-to-noise” ratio.

Back to article page