Fig. 5: Intravital tracking of the ISC-EB-EC transition using the unique pattern of EEs. | Communications Biology

Fig. 5: Intravital tracking of the ISC-EB-EC transition using the unique pattern of EEs.

From: Intravital imaging strategy FlyVAB reveals the dependence of Drosophila enteroblast differentiation on the local physiology

Fig. 5

a Representative images of the ISC-EB-EC transition. The EE pattern (E1-E5, solid line) was used to trace the same region at 8 time points over 109 h. The EEs were visualized at first by low laser power and low digital gain under the 561 nm laser line (first row). ISCs, EBs, and EEs were subsequently visualized in the same region by higher laser power and digital gain under 488 nm and 561 nm laser line (second row). Magnification and schematic illustration inside the squared area (dashed line) are shown in the third and fourth row. An ISC divided once to generate one ISC and one EB, and this EB further differentiated into an EC. The intensity of the green color indicates the amount of NRE-GFP. Genotype: esg-Gal4 10×UAS-myr:tdTomato, NRE-GFP; pros-QF2 20×QUAS-6×mcherry. Scale bars, 10 μm. b Quantification of the GFP ratio in the new generated EB (EC) during the ISC-EB-EC transition. The value of the GFP ratio is indicated at each time point.

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