Fig. 6: CryoEM single-particle reconstruction of tcTRP24₈SS.

Two different views of the electron density map superposed with the original model. The three-strong features extending from equivalent positions around the tcTRP particle correspond to N-terminal poly-histidine affinity tags and linkers extending from the N-terminus of each protein subunit (an unbiased feature that further validated the trimeric assemblage of the particle). Those atoms are clearly disordered and are not modeled into the density.