Fig. 5: Impact of Pfkfb3 knockdown on glycolysis in muscle fibers.
From: Sex differences in metabolic pathways are regulated by Pfkfb3 and Pdk4 expression in rodent muscle
a Male quadriceps muscle fibers were transfected with siPfkfb3 (n = 3) or control siRNA (n = 3) for 6, 12, or 24 h. The amount of Pfkfb3 mRNA was determined by qRT-PCR. The ratios of the amounts of Pfkfb3 mRNA in the siPfkfb3- and control siRNA-treated fibers are indicated. b The expressions of PFKFB3 and GAPDH were examined by western blotting at 6, 12, and 24 h after the siRNA transfection. The intensities of the PFKFB3 signals were normalized to those of GAPDH, and the relative amounts are presented as means ± SD (n = 3 in each group). Full blot images are shown in Supplementary Fig. 5b. c ECARs were examined in the male-derived fibers treated with control siRNA (M), male-derived fibers treated with siPfkfb3 (M-KD), and female-derived fibers treated with control siRNA (F). The fibers were treated with siRNA for 12 h (left) or 24 h (right). The data were corrected by the ratio of the mean protein content in the fibers from the two sexes. Data from three biologically independent samples were analyzed as described in the “Methods” section. The bars (means ± SD) with the same letter are not significantly different from each other (p < 0.01).
