Fig. 1: Identification of Zfhx4 as an important transcription factor for skeletal development.
From: Zfhx4 regulates endochondral ossification as the transcriptional platform of Osterix in mice
a List of highly expressed transcription factors in limb buds determined by microarray analysis. b Expression of Zfhx4 in tissues of newborn mice. Total RNA was isolated from the indicated tissues and analyzed by RT-qPCR. Data are shown as the mean ± s.d. (n = 3). c Expression of Zfhx4 in the growth plate of mice. Hematoxylin and eosin staining (HE) and in situ hybridization (ISH) were carried out on growth plate chondrocytes of femurs from E16.5 mice. Scale bar = 500 μm. d Macroscopic views of the palate of P0 wild-type (WT) and Zfhx4−/− littermates. Cleft palates were observed in Zfhx4−/− mice. Scale bar = 1 mm. e Skeletal preparations of whole embryos, ribs, mandibles, skulls, forelimbs, and hindlimbs of E15.5 WT and Zfhx4−/− littermates stained with Alcian blue and alizarin red. Scale bar = 1 mm.
