Fig. 5: Association and prediction of electrophysiological features from gene expression.

a Bibiplots for the mouse visual cortex using the NMA’s latent spaces (first three components used). Cells are dots (n = 3654). Transcriptomic and electrophysiological latent spaces are shown as columns. Each biplot shows the subspace of two components. Cells are colored by their cross-modal clusters. The line length of a gene or electrophysiological feature (i.e., radius) corresponds to its correlation with the latent space with max value = 1. The genes and electrophysiological features with correlations >0.6 are shown here. The label positions are slightly adjusted to avoid overlapping. b Similar to (a) but for the mouse motor cortex (n = 1208). c The representative electrophysiological features in the cross-modal clusters with testing \({R}^{2}\) > 0.5 (90% training set, 10% testing set, see Methods). d The predicted values by gene expression (x-axis) vs. the observed values (y-axis) of the upstroke downstroke ratio (\({R}^{2}=0.805\)) in the visual cortex and the action potential width (\({R}^{2}=0.800\)) in the mouse motor cortex.