Table 1 Data collection and processing statistics for AcrB in a cycloalkane-modified amphiphilic polymer.
AcrB (EMDB-12043) (PDB 7B5P) | |
|---|---|
Data collection and processing | |
Magnification | 130,000 |
Voltage (kV) | 300 |
Electron exposure (e–/Å2) | 58 |
Defocus range (μm) | 1.7–3.5 |
Pixel size (Å) | 1.07 |
Symmetry imposed | C1 |
Initial particle images (no.) | 40,2672 |
Final particle images (no.) | 10,0932 |
Map resolution (Å) | 3.2 |
FSC threshold | 0.143 |
Map resolution range (Å) | 3.19–6.12 |
Refinement | |
Initial model used (PDB code) | 6baj |
Model resolution (Å) | 3.3 |
FSC threshold | 0.5 |
Model resolution range (Å) | |
Map sharpening B factor (Å2) | −75 |
Model composition | |
Non-hydrogen atoms | 22527 |
Protein residues | 3153 |
Ligands | 0 |
B factors (Å2) | |
Protein | 46 |
Ligand | |
R.m.s. deviations | |
Bond lengths (Å) | 0.009 |
Bond angles (°) | 0.997 |
Validation | |
MolProbity score | 2.71 |
Clashscore | 25 |
Poor rotamers (%) | 2.27 |
Ramachandran plot | |
Favoured (%) | 90 |
Allowed (%) | 9 |
Disallowed (%) | 1 |
