Fig. 5: The characterization of the pluripotent state is evaluated after long-term expansion by different pluripotency markers. | Communications Biology

Fig. 5: The characterization of the pluripotent state is evaluated after long-term expansion by different pluripotency markers.

From: A miniature dialysis-culture device allows high-density human-induced pluripotent stem cells expansion from growth factor accumulation

Fig. 5

a The hiPSCs expanded in D/MR(−) showing higher alkaline phosphatase (ALP) activity. b This activity is also related to higher expression of intracellular ALP in the hiPSCs expanded in D/MR(−), detected by immunocytochemistry. The percentage indicated the protein expression of cytoplasmic ALP, relative to nuclear staining (DAPI). c The higher expression level of SSEA-4 surface marker and d Three main transcription factors regulating pluripotency (OCT4, SOX2, and Nanog) were detected in hiPSCs expanded in D/MR(−). (n = 4 biologically independent experiments). Mean ± standard deviation are indicated in each graph. Statistical significance: ****p < 0.0001; ***p < 0.001; **p < 0.01; *p < 0.05. e Their expression in the protein level was confirmed by immunocytochemistry of OCT3/4, SOX2, and NANOG of the aggregates from the three different hiPSC lines cultured in D/MR(−) and C/MR(+). The percentage indicated the number of positive cells, relative to nuclear staining (DAPI). The C/MR(−) was excluded from the analysis due to the insufficient cell yield.

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