Table 1 The medium formulation used in this study.
Culture medium component | Molecular weight | Volume or concentration |
|---|---|---|
1. DMEM/F12 basal medium (Life Technologies, USA) containing: ◦ Carbohydrate ◦ Amino acids ◦ Inorganic salt ◦ Vitamins ◦ Buffer ◦ Antioxidant ◦ Other small molecule components | All components <1 kDa | 2 ml (culture compartment); 15 ml (dialysate compartment) |
2. Supplemented growth factors (only administrated in culture compartment): | ||
◦ FGF2 (Life Technologies, USA) | 18 kDa | 100 ng/ml |
◦ Insulin (Life Technologies, USA) | 5.74 kDa | 19.4 µg/ml |
◦ Transferrin (Life Technologies, USA) | 80 kDa | 10.7 µg/ml |
◦ TGFβ-1 (Life Technologies, USA) | 25 kDa | 2 ng/ml |
3. FP003 (Nissan chemical, Japan) (only administrated in culture compartment) | 500 kDa | 2% |
