Fig. 1: Single-cell RNA-sequencing analysis of Tregs from HLA-B27+ ankylosing spondylitis blood and synovial fluid reveals multiple distinct clusters. | Communications Biology

Fig. 1: Single-cell RNA-sequencing analysis of Tregs from HLA-B27+ ankylosing spondylitis blood and synovial fluid reveals multiple distinct clusters.

From: Single cell analysis of spondyloarthritis regulatory T cells identifies distinct synovial gene expression patterns and clonal fates

Fig. 1: Single-cell RNA-sequencing analysis of Tregs from HLA-B27+ ankylosing spondylitis blood and synovial fluid reveals multiple distinct clusters.

a Experimental design of scRNA-seq of Tregs including gene expression and V(D)J TCR gene segment sequencing. PBMCs and synovial fluid mononuclear cells (SFMCs) from two patient with active HLA-B27+ AS were sampled during an arthritis flare. Figure generated using Biorender. b Reduced dimensionality visualization (UMAP plot) and clustering of the transcriptome of 13,397 Tregs from peripheral blood and synovial fluid. c Cell numbers from peripheral blood and synovial fluid and fractions composing each tissue. The asterisks indicate clusters enriched in either peripheral blood (CCR7+ and KLRB1+) or synovial fluid (all the others) (*p < 0.05, z-test of two proportions). d Expression (scaled log(UMI + 1)) of lineage-defining markers FOXP3 and IL2RA across various clusters, sorted by average expression from the highest-expressing cluster. e Heatmap of row-wise z-score-normalized mean expression of selected marker genes, chosen among top differentially expressed for each cluster and other known markers. f Distribution of genes with known T-regulatory function across various clusters. Dotplot heatmap showing average scaled expression (color) and percentage of cells (dot size) expressing the genes, split into highly expressed (left panel) and cluster-specific (right panel).

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