Fig. 1: INS-1 acts in both DAF-2c and DAF-16 pathways in taste avoidance learning.
a Schematic of taste avoidance learning. b Salt chemotaxis after conditioning on agar plates at high or low salt concentrations in the absence of food. A chemotaxis index was determined according to the following equation: Chemotaxis index = (NA − NB)/(Nall − NC), where NA and NB are the number of worms in the high- and low-salt areas, respectively, Nall is the total number of worms on a test plate, and NC is the number of worms in the area around the starting position. Positive and negative chemotaxis indices mean migration toward high and low salt, respectively. Each dot in red or blue represents a chemotaxis index calculated in each chemotaxis assay after conditioning at high or low salt concentrations, respectively. n = 4 assays. Bars represent mean values; error bars represent SEM. Two-tailed Welch’s t-test with Holm correction: *P < 0.05 and **P < 0.01. ns not significant. c Proposed genetic pathways downstream of ins-1 in the regulation of low- or high-salt migration after high- or low-salt conditioning in the absence of food, respectively.
