Fig. 7: Identification of a TWIST1-mediated-tumor-stroma signature associated with poor outcome in NB. | Communications Biology

Fig. 7: Identification of a TWIST1-mediated-tumor-stroma signature associated with poor outcome in NB.

From: TWIST1 expression is associated with high-risk neuroblastoma and promotes primary and metastatic tumor growth

Fig. 7: Identification of a TWIST1-mediated-tumor-stroma signature associated with poor outcome in NB.

a Bar plot illustrating of the 77 DE genes representing the TWIST1-tumor-stroma signature in SK-N-Be2c ortho_1 tumors. Genes were classified according to their log2(FC) in three main categories: growth factors (including the TGF and FGF families) cytokines (TNF and IFN families, chemokines and interleukins), and integrins and their ligands (ITG, collagens and laminins). b Kaplan−Meier OS and EFS curves of NB patients of the SEQC dataset according to the expression level of the TWIST1-tumor-stroma signature. Expression cutoff for both curves: 0.10. c Volcano plot showing the distribution of the DE protein secreted by SK-N-Be2c cells according to the delta label-free quantification (∆LFQ = LFQ SK-N-Be2c Control – LFQ SK-N-Be2c sgTWIST1) intensities (Log2) and the adjusted p values with an FDR ≤ 0.02 analyzed by LC-MS/MS (n = 3 biological replicates for each group). d 3D scatterplot showing DE terms in the cell secretome in common with the tumor transcriptome (magenta, n = 55), the cell transcriptome (green, n = 75), or both transcriptomes (blue, n = 131). e Bar plot showing the terms commonly deregulated between the TWIST1-tumor-stroma signature and both the cell transcriptome and secretome. Names in brackets are for terms found to be DE in the secretome but not in the transcriptome of cells.

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