Fig. 3: Characterization of the melanocyte compartment after 2 weeks in culture.
From: Human immunocompetent choroid-on-chip: a novel tool for studying ocular effects of biological drugs
Morphology of pigmented melanocytes seeded at low (a, top) and high (b, bottom) densities and embedded in the dextran hydrogel containing Arginylglycylaspartic acid (RGD peptide): Bright field images demonstrate pigmentation; autofluorescence shows melanin, FDA/PI labeling indicates viability (Epifluorescence imaging of the whole chip), and KI-67 staining (Confocal imaging) a low proliferative state. Scale bar: 500 µm (Ki-67 images: 100 µm); (c) 3D representation of the cell distribution in the melanocyte compartment (left). Immunofluorescence microscopy highlighting pigmentation (bright filed; top left), melanin autofluorescence (top right), cell nuclei (DAPI; bottom left) and the presence of TYRP1 (bottom right) (Confocal imaging). d Effect of melanocyte density on the cytokine secretion in the presence of peripheral blood mononuclear cells (PBMCs). The bars represent the average values and the dots the individual values (n = independent biological replicates). Statistical analysis is detailed in Supplementary data 1, including individual data points.
