Fig. 3: ROS and GA content in the GSNOR-silenced strains treatment with 3-AT under HS conditions. | Communications Biology

Fig. 3: ROS and GA content in the GSNOR-silenced strains treatment with 3-AT under HS conditions.

From: GSNOR regulates ganoderic acid content in Ganoderma lucidum under heat stress through S-nitrosylation of catalase

Fig. 3

a Coverslips were placed on the bottom Petri dishes; after the WT and GSNOR-silenced strains cultured on mycelium grew onto the coverslips, the fungal mycelium was transferred onto a PDA plate with 3-amino-1,2,4-triazole (3-AT) for 30 min, then exposed to 42 °C for 20 min. ROS content was measured by staining with DCFH-DA in the WT and GSNOR-silenced strains untreated or treated with 3-AT. Scale bar, 100 μm. b ROS levels in the WT and GSNOR-silenced strains treated with 3-AT. c The WT and GSNORi strains were grown for 5 days in PDA liquid cultures with shaking and then treated with 3-AT for 30 min. The strains were exposed to 42 °C for 12 h and shifted to 28 °C until the 7th day in stationary. The GA content in the GSNOR-silenced strains was untreated or treated with 3-AT in the heat-stressed strains. Data are presented as the mean ± SD of data from three independent experiments (**P < 0.01, ***P < 0.001; ****P < 0.0001 by two-way ANOVA).

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