Fig. 4: ROS and GA content in the CAT-silenced strains treatment with GSNO under HS conditions.

a Coverslips were placed on the bottom Petri dishes; after the WT and CAT-silenced strains cultured on mycelium grew onto the coverslips, the fungal mycelium was transferred onto a PDA plate with S-nitrosogluthionine (GSNO) for 30 min, then exposed to 42 °C for 20 min. ROS content was measured by staining with DCFH-DA in the WT and CAT-silenced strains untreated or treated with GSNO. Scale bar, 100 μm. b ROS levels in the WT and CAT-silenced strains treated with GSNO. c The WT and CATi strains were grown for 5 days in PDA liquid cultures with shaking and then treated with 3-AT for 30 min. The strains were exposed to 42 °C for 12 h and shifted to 28 °C until the 7th day in stationary. The GA content in the CAT-silenced strains was untreated or treated with GSNO in the heat-stressed strains. Data are presented as the mean ± SD of data from three independent experiments (**P < 0.01, ***P < 0.001; ****P < 0.0001 by two-way ANOVA).