Fig. 5: S-nitrosylation of the CAT protein in vitro and in vivo. | Communications Biology

Fig. 5: S-nitrosylation of the CAT protein in vitro and in vivo.

From: GSNOR regulates ganoderic acid content in Ganoderma lucidum under heat stress through S-nitrosylation of catalase

Fig. 5

a Recombinant CAT was treated with GSH (negative control for GSNO) or GSNO to measure S-nitrosylation by the biotin switch method. Without sodium ascorbate (Asc) sample as another negative control. Protein samples were separated via SDS–PAGE and analyzed by immunoblotting using an anti-biotin antibody and the His tag antibody. b Protein extracts were prepared from WT and GSNOR-silenced strains, and the proteins were analyzed by the biotin-switch assay. The protein was incubated with streptavidin beads, which were then washed to detect the S-nitrosylation of the CAT protein in the WT and GSNOR-silenced strains. Equal loading was verified by antibodies specific for actin and CAT.

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