Fig. 3: Cone cytoskeleton and inner/outer segment alignment.

3D reconstructions (a–k, reconstructed volumes in a–f, h, and j), and an optical slice (l) of cones immunolabeled for espin 1 (magenta in all reconstructed volumes: a, b, d, j; blue in fluorescence 3D image I and magenta in fluorescence 3D image k), Protocadherin 15 (pcd15, green), acetylated tubulin alpha (tuba, blue in all reconstructed volumes: c, e, f, h, j; red in fluorescence 3D images in g and i, green in k and l) rootletin (CROCC, orange in all reconstructed volumes: c, e, f, h, and j, blue in fluorescence 3D image g and yellow in fluorescence 3D image i). a, b, d and c, e, f represent three perpendicular reconstitutions of the proteins. g yellow arrowheads underline the rootletin anchor. An optical slice in the mid-inner segment is shown for a single cone in l, the alternation between espin 1 and tubulin is readily visible both visually and on the fluorescence profile (m, numerical data in supplementary data 3). OS outer segment, CP calyceal processes, IS inner segment, N nucleus. Scale bars represent 0.5 µm in (c), 1 µm in (a, d, e), and 2 µm in (g, I, and k).