Fig. 1: SilThPOK represses gene expression at the DP stage in CD4ThPOK.Sil knock-in mice.
From: An autonomous TCR signal-sensing switch influences CD4/CD8 lineage choice in mice
a Schematic of Cd4 gene organization in wt mice (top row), CD4ΔSil (second row) or CD4ThPOK.Sil knock-in mice (bottom row). Black boxes indicate exons. Enhancers are shown as white boxes, the Cd4 silencer as a red circle, and the ThPOK silencer as a red triangle. b FACS analysis of CD4, and CD8a expression of total thymocytes (top row), or indicated gated thymocyte subsets (bottom 2 rows) of CD4Δsil/Δsil, CD4O/ThPOK.Sil, CD4O/O, and wt mice. Note that CD4 expression is severely reduced at immature DP-like stage, but is substantially upmodulated in many CD69+ TCRβ+ thymocytes that have received a recent TCR signal. c FACS analysis of CD4 and CD8a expression of total mesenteric lymph node (LN) cells (top row), or gated TCRβ+ LN cells subsets (bottom row) of same strains of mice as above. Results are representative of three independent experiments (n = 3, for each strain). d Plots showing % of DP, SP CD4, SP CD8, and DN thymocytes for mice of indicated genotypes. N = 3 independent animals. Data are presented as mean values +/− SEM. A P value < 0.05 was considered significant. Significant differences were determined by one-way ANOVA with post hoc Tukey HSD (honest significant difference), and indicated by asterisks (*P < 0.01; **P < 0.005; ***P < 0.001). Statistical significance was calculated for each indicated mutant line relative to wt mice.
