Fig. 7: Mutation of EGR and NFAT-binding motifs adjacent to conserved Runx sites impairs SilThPOK function.
From: An autonomous TCR signal-sensing switch influences CD4/CD8 lineage choice in mice
a EMSA analysis showing EGR1 and NFAT2 binding to oligos comprising wt silencer sequence (region surrounding Runx sites), or mutated at consensus EGR or NFAT motifs, as indicated. b Position of TF consensus binding sites for wt silencer, or indicated variant alleles. FACS analysis of TCRβ, CD69, CD4, and CD8 expression of total thymocytes, gated thymocytes (c), and peripheral T-cell subsets (d) of wt mice or mutant lines, as indicated. Results are representative of multiple experiments. N = 6 independent animals per strain. Data are presented as mean values +/− SEM. A P value < 0.05 was considered significant. Statistical significance was determined between indicated mutant mice and ThPOK+/+ mice by one-way ANOVA with post hoc Tukey HSD, and indicated by asterisks (*P < 0.01; **P < 0.005; ***P < 0.001). Statistical significance was calculated for each indicated mutant line relative to wt mice.
