Fig. 8: Mutation of EGR and NFAT-binding motifs adjacent to conserved Runx sites impairs normal lineage choice of MHC class I-restricted thymocytes. | Communications Biology

Fig. 8: Mutation of EGR and NFAT-binding motifs adjacent to conserved Runx sites impairs normal lineage choice of MHC class I-restricted thymocytes.

From: An autonomous TCR signal-sensing switch influences CD4/CD8 lineage choice in mice

Fig. 8: Mutation of EGR and NFAT-binding motifs adjacent to conserved Runx sites impairs normal lineage choice of MHC class I-restricted thymocytes.

a, b FACS analysis of TCRβ, CD69, CD4, and CD8 expression of total thymocytes, gated thymocytes (a), and peripheral T-cell subsets (b) of wt, or mutant mouse lines crossed to MHC II−/− background. c, d Plots showing % of DP, SP CD4, SP CD8, and DN thymocytes for mice of indicated genotypes. N = 6 independent animals per strain. Data are presented as mean values +/− SEM. A P value < 0.05 was considered significant. Statistical significance was determined between mutant mice and ThPOK+/+ mice on MHC II−/− background by one-way ANOVA with post hoc Tukey HSD, and indicated by asterisks (*P < 0.01; **P < 0.005; ***P < 0.001). Statistical significance was calculated for each indicated mutant line relative to wt mice.

Back to article page