Fig. 1: Transient hyperglycemia causes persistent suppression of RORA and its target genes during subsequent normoglycemia. | Communications Biology

Fig. 1: Transient hyperglycemia causes persistent suppression of RORA and its target genes during subsequent normoglycemia.

From: Maternal diabetes-mediated RORA suppression in mice contributes to autism-like offspring through inhibition of aromatase

Fig. 1

Human ACS-5003 neurons were treated with either 5 mM low glucose (LG) or 25 mM high glucose (HG) for 4 days. The cells were then infected by empty (CTL), RORA overexpression (↑RORA), or RORA knockdown (shRORA) lentivirus for one day before treatment with LG for another 4 days in the presence of 1% serum; the cells were then harvested for further analysis. a, b Cells were harvested at different time points for analysis of mRNA levels. a RORA levels; b CYP19A1 levels; n = 4, *, P < 0.0001, vs. day 0 group; ¶, P < 0.0001, vs. day 4 group. c–g Cells were harvested on day 8 for biomedical analysis. c mRNA levels, n = 4. d Quantitation of protein levels, n = 5. e Representative western blotting pictures for (d). f ROS formation, n = 5. g 3-nitrotyrosine formation, n = 5. *, P < 0.0001, vs. LG(4d)+LG(4d)/CTL group. Data were expressed as mean ± SEM.

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