Fig. 6: Postnatal expression of RORA in the amygdala reverses maternal diabetes-induced gene expression and oxidative stress in offspring, while RORA knockdown mimics this effect. | Communications Biology

Fig. 6: Postnatal expression of RORA in the amygdala reverses maternal diabetes-induced gene expression and oxidative stress in offspring, while RORA knockdown mimics this effect.

From: Maternal diabetes-mediated RORA suppression in mice contributes to autism-like offspring through inhibition of aromatase

Fig. 6

The male offspring from either the control (CTL) or maternal diabetes (STZ) groups received either vehicle (P-VEH), or lentivirus infusion for either RORA expression (P- ↑ RORA) or RORA knockdown (P-shRORA) at 6 weeks old, and the male offspring were sacrificed for further biomedical analysis at 8 weeks old. a–d The amygdala tissues were isolated for further analysis as described as follows: a mRNA levels by qPCR, n = 4. b The quantitation of protein levels, n = 5. c The representative pictures for western blotting. d CSF E2 levels, n = 5. e Estrogen receptor activity, n = 5. f In vivo superoxide anion release, n = 5. g The amygdala tissues were isolated for the measurement of 8-OHdG formation, n = 5. *, P < 0.0001, vs. CTL/P-VEH group; ¶, P = 0.002, vs. STZ/P-VEH group. Data were expressed as mean ± SEM.

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