Fig. 2: Production of HGF mimetic STaMPtides.
From: Single-chain tandem macrocyclic peptides as a scaffold for growth factor and cytokine mimetics
a Chemical structure of HGF mimetic aMD4dY-PA22. b SDS-PAGE analysis of secreted linker variants of HGF mimetic STaMPtides. Red dots indicate STaMPtide-derived bands. M: marker, NC: negative control supernatant (mock-vector-transfected). c Identification of disulfide bonds of aMD4dY-PA22. aMD4dY-PA22 was submitted for thermolysin digestion without reductive alkylation, and peptide fragments were analyzed by LC–MS/MS. The base peak chromatogram of 5–15 min is shown, and peaks of disulfide-containing fragments are highlighted in green boxes.
