Fig. 4: Bioactivity evaluation of EPO mimetic STaMPtides.
From: Single-chain tandem macrocyclic peptides as a scaffold for growth factor and cytokine mimetics
a Signaling potencies induced by EMP-PA8 and EMP-PA22. EPOR-JAK2 signaling activation by EMP-PA8 (purple squares), EMP-PA22 (red triangles), rhEPO (blue circles), and monomeric EMP35 (green inverted triangles) was evaluated by EPOR-JAK2 functional reporter assay. The mean and individual values are shown. b Colony-forming cell assay. hPBMCs were cultured in methylcellulose culture medium in the absence (black bar) or presence (blue bar) of 0.54 nM rhEPO or 1.6–16 nM EMP-PA22 (red bars). Number of erythroid and myeloid colonies formed from 2 × 105 hPBMCs were counted. The mean ± SD with individual values are shown from the results of quadruplicate experiments. ****p < 0.0001. c Morphology of erythroid colonies formed in methylcellulose medium in the presence of 0.54 nM rhEPO or 16 nM EMP-PA22 after 13 days of culture. Scale bars = 200 μm.
