Fig. 1: Removal of extracellular Ca2+ triggers basal Ca2+ drop in intracellular compartments and affects ER-PM contact cites.
From: Citrin mediated metabolic rewiring in response to altered basal subcellular Ca2+ homeostasis
a Removal of extracellular Ca2+ reduces basal Ca2+ levels in cytosol (n = 64), ER (n = 17), IMS (n = 13), and mitochondria (n = 21). b Exemplary 3D reconstruction of EA.hy926 cell with pseudo- colored ER (green) and mitochondria (red) used for analysis of ER-mitochondria contacts (dark blue). c Analysis of ER-mitochondria contacts (n = 22, unpaired t-test; n.s.; p = 0.68). d Exemplary TIRF images of ER in the plasma membrane proximity in 2 Ca2+ (left) and 0 Ca2+ (right) conditions, scale bar (left bottom corner) is 5 µm. e Statistical analysis of ER-PM contacts (unpaired t-test, n = 9; n.s. p = 0.92,; *p = 0.018; ***p = 0.004).
