Fig. 5: Citrin KD mimics the metabolic alterations happening during basal Ca2+ drop condition.
From: Citrin mediated metabolic rewiring in response to altered basal subcellular Ca2+ homeostasis
a Basal cytosolic lactate levels of control vs Citrin KD in 2 Ca2+ (unpaired t-test, n = 7; *p = 0.0411). b Lactate accumulation after extracellular Ca2+ removal (as in Fig. 4 a) in control vs Citrin KD (unpaired t-test, n = 15 for control, n = 13 for citrin KD; **p = 0.0064). c Basal cytosolic NADH/NAD+ ratio of control vs Citrin KD in 2 Ca2+ (unpaired t-test, n = 30 for control, n = 18 for citrin KD; ***p = 0.0005). d NADH/NAD+ ratio change after extracellular Ca2+ removal (as in Fig. 4 e) in control vs Citrin KD (unpaired t-test, n = 29 for control, n = 18 for citrin KD; **p = 0.0002). e Average traces (solid lines) and single data points of mitochondrial NADH autofluorescence measurements in control and Citrin KD cells. f Statistical comparison of basal mitochondrial NADH in control vs Citrin KD shown in panel e (unpaired t-test, n = 11; **p = 0.0064). g Maximal NADH production in control vs Citrin KD cells shown in panel e (unpaired t = test, n = 11; *p = 0.0333).
