Fig. 8: Mitochondrial bioenergetics are influenced by basal MAM Ca2+ flux.
From: Citrin mediated metabolic rewiring in response to altered basal subcellular Ca2+ homeostasis
a Basal matrix ATP levels in control cells and cells incubated with 50 µM BAPTA-AM or 50 µM EGTA-AM for 30 min (One-Way ANOVA with Tukey’s multiple comparison test, ***p < 0.001, n = 32 for control, n = 14 for BAPTA-AM, n = 11 for EGTA-AM). b Analysis of mitochondrial ATP change after 2 µM oligomycin addition to cells perfused with 2 Ca2+ or 0 Ca2+, and pre-incubated with 50 µM BAPTA-AM or 50 µM EGTA-AM (One-Way ANOVA with Tukey’s multiple comparison test, *p < 0.05, **p < 0.01, ***p < 0.001, n = 13 for 2 Ca2+, n = 19 for 0 Ca2+, n = 14 for BAPTA-AM, n = 11 for EGTA-AM). c Analysis of mitochondrial ATP change after 2 µM oligomycin addition to cells incubated with DMSO control (n = 31), 3 µM Pyr6 (n = 17), 3 µM Pyr10 (n = 15), 10 µM XeC (n = 24), and 3 µM Pyr6/3 µM Pyr10 (n = 17), One-Way ANOVA with Tukey’s multiple comparison test, **p < 0.01, ***p < 0.001. d Basal matrix ATP levels in control, IP3R2 KD and Orai1 KD cells (One-Way ANOVA with Tukey’s multiple comparison test, ***p < 0.001, *p < 0.05, n = 20 for control, n = 29 for IP3R2 KD, n = 25 for Orai1 KD). e Analysis of mitochondrial ATP change after 2 µM oligomycin addition to control, IP3R2 KD and Orai1 KD cells (One-Way ANOVA with Tukey’s multiple comparison test, *p < 0.05 versus control, n = 20 for control, n = 29 for IP3R2 KD, n = 25 for Orai1 KD).
