Fig. 2: Li⁺ suppresses expressions of NLRP3 and GSDMD induced by ischaemia-reperfusion.

Cortices of Thy1-YFP and GFAP-GFP transgenic mice were used for isolating and FACS sorting neurones and astrocytes, respectively, for FACS sorting of microglia the cells from cortex of wild-type mice were labelled with Iba1. a The representative protein bands of NRLP3, caspase-1, GSDMD, pro-caspase-1, ASC and β-actin (the house-keeping protein) in neurones, astrocytes and microglia. b–g Normalised intensities of NLRP3, caspase-1, GSDMD, pro-caspase-1 and ASC by β-actin and the ratio of caspase-1 and pro-caspase-1 are presented as mean ± SD. N = 6 per group. One-way ANOVA for comparisons including more than two groups; unpaired two-tailed t-test for two group comparisons. *p < 0.05, **p < 0.01, ***p < 0.001.