Fig. 4: The effects of Li+ in the nuclear levels of β-catenin, FoxO3a and TCF4 by stimulating AKT following ischaemia-reperfusion.
From: The neuroprotective mechanism of lithium after ischaemic stroke
a The representative protein bands of β-catenin, FoxO3a and Histone H3 (the house-keeping protein) in nucleus. The normalised intensities of β-catenin and FoxO3a by Histone H3 were shown in b and c, inserts on the right are the relative comparison with the respective MCAO groups. d The representative protein bands of β-catenin, TCF4 and Histone-H3 (the house-keeping protein) in nucleus. The binding ratio of TCF4 with β-catenin is shown in e, and the normalised intensities of Histone H3 by β-actin are shown in f, the right insert shows comparison with the respective MCAO groups. The protein levels were calculated and plotted as mean ± SD. N = 6 per group. Two-way ANOVA for comparisons including more than two groups; unpaired two-tailed t-test for two group comparisons. *p < 0.05, **p < 0.01, ***p < 0.001.
