Table 1 Dissociation and rate constants for the interaction of spike hepta-peptides with COPI-WD40 domains.

From: An extended motif in the SARS-CoV-2 spike modulates binding and release of host coatomer in retrograde trafficking

Peptide sequencea

COPI-WD40 analyteb

pH

Equilibrium KD (µM)

Kinetic KD (µM)

kon (103/Ms)

koff (10−1/s)

GVKLHYT

α

7.5

4.17 ± 0.04

2.75 ± 0.09

89.96 ± 2.72

2.50 ± 0.03

GVKLHYT

β‘

7.5

n.d.

n.d.

n.d.

n.d.

VLKTYHG

α

7.5

n.d.

n.d.

n.d.

n.d.

GVKLKYT

α

7.5

4.17 + 0.31

2.71 + 0.05

67.3 + 1.45

1.84 + 0.01

GVKLKYT

β‘

7.5

n.d.

n.d.

n.d.

n.d.

GVKLHYT

α

6.5

1.40 ± 0.15

0.95 ± 0.02

152.13 ± 3.45

1.45 ± 0.01

GVKLHYT

β‘

6.5

n.d.

n.d.

n.d.

n.d.

GVALHYT

α

6.5

n.d.

n.d.

n.d.

n.d.

GVKLAYT

α

6.5

n.d.

n.d.

n.d.

n.d.

GVALAYT

α

6.5

n.d.

n.d.

n.d.

n.d.

GVKLHAT

α

6.5

3.77 ± 0.34

2.32 ± 0.07

120.87 ± 3.39

2.80 ± 0.03

GVKLHYA

α

6.5

1.13 ± 0.05

1.14 ± 0.02

66.67 ± 0.80

0.76 ± 0.01

GVKLHYV

α

6.5

3.20 ± 0.04

2.06 ± 0.05

102.67 ± 2.61

2.12 ± 0.02

GVKLHYE

α

6.5

0.31 ± 0.01

0.38 ± 0.01

58.96 ± 0.54

0.24 ± 0.003

GVKLHYQ

α

6.5

0.78 ± 0.04

1.15 ± 0.01

60.05 ± 0.75

0.66 ± 0.01

GVKLHYR

α

6.5

1.73 ± 0.24

1.29 ± 0.04

57.60 ± 0.61

1.77 ± 0.01

KEVYLHG

α

6.5

n.d.

n.d.

n.d.

n.d.

GVKLHYT

α-Ala57

6.5

n.d.

n.d.

n.d.

n.d.

GVKLHYT

α-Ala115

6.5

n.d.

n.d.

n.d.

n.d.

GVKLHYT

α-Ala139

6.5

n.d.

n.d.

n.d.

n.d.

  1. aThe sequences of immobilized sarbecovirus spike peptides are given in the left column. The dibasic motif residues are highlighted in bold. Mutations in the peptides relative to the wild-type sequence are highlighted in bold and are underlined.
  2. bα and β’ represent wild-type αCOPI-WD40 and β’COPI-WD40, respectively. α-Ala57, α-Ala115, and α-Ala139, represent the Arg57 → Ala, Asp115 → Ala, and Tyr139 → Ala mutants of αCOPI-WD40, respectively.
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