Table 1 Plasmids used in this study.
Plasmid | Description |
|---|---|
pME | Mammalian cell expression vector |
YEp352GAPII | URA3/2μ yeast shuttle vector containing TDH3 promoter |
pME-FLAG-hALG2 | FLAG-hAlg2 expressed in pME |
pME-GFP | Enhanced GFP expressed in pME |
pME-GFP-hALG2 | GFP-hAlg2 expressed in pME |
pME-hALG2-GFP | hAlg2-GFP expressed in pME |
pME-mRFP-KDEL | mRFP-KDEL expressed in pME |
YEp352GAPII-FLAG-SUC2A-hALG2 | FLAG-Suc2A-hAlg2 expressed in YEp352GAPII |
YEp352GAPII-hALG2-SUC2A-FLAG | hAlg2-Suc2A-FLAG expressed in YEp352GAPII |
